Fluorescence Quantitation
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Compensation in Flow Cytometry
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HOW OFTEN SHOULD I RUN COMPENSATION? #AskExCyte Compensation is a critical parameter to get correct for proper interpretation of flow cytometry data. It address the physics of fluorescence and the spectral spillover of a fluorochrome into a secondary channel. Compensation should be performed with every experiment. It is not best practices to re-use a compensation matrix from day-to-day or week-to-week. To ensure the best quality compensation, follow the three rules of compensation and remember t
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