The cssDNA from this phage was isolated using silica column purification and showed 88% cssDNA purity according to agarose gel imaging (Figure 1d), with an approximate yield of 0.5 mg per liter of …

Phagemids and M13 What is phagemid? A phagemid or phasmid is a hybrids plasmid that contains an f1 origin of replication from an f1 phage. It can be used as a type of cloning vector in combination …

(b) a nucleic acid helper plasmid for expressing a plurality of bacteriophage proteins capable of assembling a single-stranded DNA (ssDNA) into a bacteriophage.

Helper plasmid for production of phagemid ssDNA, based on m13mp18

This chapter discusses the production of single-stranded plasmid DNA. The chapter focuses on M13KO7 and its uses. The chapter reviews M13 biology; M13 mutants play a vital role in the …

Single-stranded (ss)DNA is a pre- ferred template for dideoxy DNA se- quencing. It is most commonly ob- tained by cloning the studied sequences into an M13 vector (1); however, large inserts tend to be …

Nov 16, 2022 · Background Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded DNAs with a DNA lesion, …

M13 occurs in both single and double stranded forms RF can be digested with restriction endonucleases inserts can be cloned in – like plasmid “+” strands from phage particles – convenient source of single …

Single-stranded DNA stains less brightly with ethidium bromide and migrates differently than double-stranded DNA. Yield should be >50 μg single-stranded phagemid for pUC origin vectors.

For filamentous M13 phage replication, single-stranded phage DNA enters the cytoplasm upon infection and is converted into double-stranded replicative form (RF) by host enzymes.

Mar 18, 2024 · Abstract Long single-stranded DNA (ssDNA) is a versatile molecular reagent with applications including RNA-guided genome engineering and DNA nanotechnology, yet its production …

The phagemid DNA of interest, carrying the M13 origin of replication and the packaging signal, was introduced into bacterial cells maintaining the modified VCSM13 DNA as a plasmid, and the ss ...