May 10, 2025 · 1. Introduction Intracellular delivery of functional proteins to live cells holds tremendous potential for the development of therapeutics and diagnostics, such as understanding protein-protein …

Aug 7, 2025 · Graham et al. show that proximity-assisted photoactivation (PAPA) can visualize interactions of endogenous proteins at single-molecule resolution in live human cells. Applying PAPA …

Aug 15, 2024 · Lay Summary Visualizing molecular-scale interactions in living cells is challenging, but advances in single-molecule super-resolution microscopy enable high-resolution imaging of …

There are various methods to monitor protein-protein interactions without using cells, but these models do not precisely reflect the intracellular environment. Further, some PPI monitoring methods using …

Aug 17, 2022 · Proximity-assisted photoactivation (PAPA) provides a new way to detect protein–protein interactions in single-molecule imaging of live cells.

Nov 11, 2023 · Keywords: membrane protein, protein−protein interaction, super-resolution microscopy, live-cell imaging, LIVE-PAINT, yeast The ability to visualize proteins in their native cellular …

2. Fluorescence imaging of PPI The (imaging) techniques used to detect or quantify PPI need to be sensitive within the concentration ranges at which proteins are present in cells or tissues, where …

Live-cell single-molecule fluorescence imaging has become a powerful analytical tool to investigate cellular processes that are not accessible to conventional biochemical approaches. This has greatly …

Introduction Most proteins function by interacting with other proteins, yet we lack tools to study these poten-tially transient interactions at single- molecule resolution in live cells. Single- particle tracking …

Super-resolution microscopy has revolutionized biological imaging, enabling the visualization of structures at the nanometer length scale. Its application in live cells, however, has remained …